Cours de biochimie structurale: Lipides et dérivés isopréniques, Volume 4. Front Cover. Pierre Louisot. Simep éditions, – 93 pages. Cours de biochimie. Biochimie Métabolique: Cours de Cycle de Krebs Cours de la physiologie cardiaque et lymphatique svi s5 Cours de Biochimie structurale svi s3. بسم الله . Spécialité: Biochimie structurale. Présenté par. Guillaume sur l’étude de la paraoxonase humaine hPON1 au cours du service militaire du. Pr. Eric Chabrière.

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For this reason, post-translational modifications of such residues, like O -glycosylations, may be responsible for the mass shift, but this point needs further research. Strutcurale, other metabolites of the inhibitor were also formed, inhibiting other glycosylation enzymes Zanetta et al. At this time, the maturation of the protein precursor into the mature glycosylated form was nearly complete and only the mature form was detergent insoluble.

Western blot analysis The samples were processed as previously described Gouyer et al.

Detergent-insoluble GPI-anchored proteins are apically sorted in fischer rat thyroid cells, but interference with cholesterol or sphingolipids differentially affects detergent insolubility and apical sorting. The apical delivery was inhibited in the galectinKD cells, whereas the basolateral VSV-G protein was transported normally to the basolateral membrane Structurqle. Costaining experiments with different organellar markers in galectinKD cells showed partial colocalization of the cargo with lysosome-associated membrane protein 2 not depicted.

Galectin-4 and sulfatides in apical membrane trafficking in enterocyte-like cells

December 5 at 8: S2 shows the three galectin-4 RNAi constructs biofhimie Complete 1H and 13C NMR assignment of mono-sulfated galactosylceramides with four types of ceramides from human kidney.

Functional rafts in cell membranes. To enter the program, students must have a solid educational background in biology, and a good understanding of spoken and written English. Refined prognostic evaluation in colon carcinoma using immuno-histochemical galectin fingerprinting. You must fill in the information sheet available on March and then 2 send the completed application including: In conclusion, this work supplies a method for the identification of proteins secreted by LAB, allowing discrimination between the proteins present in the MRS and those produced by probiotic LAB.



N-glycans as apical sorting signals in epithelial cells. Glycosphingolipid-enriched, detergent-insoluble complexes in protein sorting in epithelial cells. Successful applicants must have a solid background in biology studies with an excellent understanding of written and spoken English AND French and will have preferably undertaken an internship of at least two months in a research laboratory.

Galectin-4 belongs to the galectin family of lectins, currently constituted of 15 individual members Dunphy et al. Four proteins secreted by the strain L. For unidentified bands, it seems that databases do not yet contain homologs of such proteins.

Identification and characterization of the novel LysM domain-containing surface protein Sep from Lactobacillus fermentum BR11 and its use as a peptide fusion partner in Lactobacillus and Lactococcus. Ten of them biohimie successfully identified Table 1and nine not identified labeled with asterisks in Fig. B Overlay experiments on the four fractions corresponding to the galactosylceramides and sulfatides purified from human sciatic nerve.

Galectin-4 could recycle from the apical membrane raft bound to bjochimie raft clustering during biosynthetic transport.

El-kouach Amine December 5 at 8: This attachment may have been removed or the person who shared it may not have permission to share it with you. Applications must be sent. Molecular and phenotypical characterization of Cronobacter species isolated with high occurrence from oats and linseeds.

To enter the program, students must have a solid background in biology, and a good command of spoken and written English for a direct application in the second year – M2 or English AND FRENCH for an application in the first year.


Soluble proteins produced by probiotic bacteria regulate intestinal epithelial cell survival and growth. This protocol allowed the steucturale of 19 proteins after sodium dodecyl sulfate-polyacrylamide gel electrophoresis, 10 of them being successfully identified by tandem MS. HT clone 5M12 cells were cultured as previously e3 Delacour et al.

B Western blotting of galectin-4 in carrier vesicles. Statistical optimization of medium components for enhanced acetoin production bichimie molasses and soybean meal hydrolysate. Structural determinants structuralle for apical sorting of an intestinal brush-border membrane protein. Previously, we have demonstrated that proteins assumed to be part of the apical delivery mechanism, syntaxin 3 and annexin XIIIb, also accumulated intracellularly after inhibitor treatment Delacour et al.

Carbohydrate-mediated Golgi to cell surface transport and apical targeting of membrane proteins.

In all cases, you will need to provide: Future work will have to define how this post-Golgi circuit operates and what path apical cargo takes and where it meets with recycling machinery.

Distinct transport vesicles mediate the delivery of plasma membrane proteins to the apical and basolateral domains of MDCK cells.

This extracellular galectin-4 staining disappeared if cells were treated with trypsin from the outside unpublished data. S1 shows NMR spectroscopy analysis of the galectin-4 ligands, i. Secreted proteins are transported from the bacterial cytoplasm to the bacterial environment.

Results showed that these ligands corresponded to sulfatides and galactosylceramides showing a similar composition stricturale those identified after co-immunoprecipitation from HT 5M12 cells with the anti—galectin-4 antibody.